Measurement

Part:BBa_K1987000

Designed by: Caroline Blassick   Group: iGEM16_UIUC_Illinois   (2016-10-14)


GFP with BsaI sites for promoter insertion

This testing construct consists of a degradation-tagged GFP orf preceded by the lac promoter and RBS. The promoter and RBS are flanked by BsaI sites, allowing for different promoter+RBS combinations to be easily swapped into the construct. The strength and expression profile of the promoter/RBS combo can then be easily measured.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 353
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 293
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None