DNA

Part:BBa_K1986001

Designed by: Carolina Guadalupe Cruz Núñez, Alejandro Alarcón del Carmen, Franklin Cruz Villegas   Group: iGEM16_Pumas_Mexico   (2016-10-14)


Optimizer in lipid production

DGTT1 from Chlamydomonas reinhardii.


===Usage and Biology===


DGTT1 genes is from Chlamydomonas reinhardii, this gene controls the syntesis of fatty acids; this biobrick is designed to express a overproduction of lipids in algae while keeping normal growth rates. The biobrick uses and Pcambia vector and 35S promoter.

It was transformed using electroporation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 829
    Illegal NotI site found at 7
    Illegal NotI site found at 740
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 307
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
       1 atgaaccaac tagagctcgt gatgtgtcga ttagctgtgt cttggttcct aatcacagct
      61 ataagccaag catgggtgtg gcctctgctc atcggcacat tgctttacgt gcagagcacc
     121 acgctcacaa ttgccttcct gctgtacctc tattatgttg tcgtcggccc gggctctaaa
     181 gatgacgcca actgcaagtg gaagccgacc ttccgcaagt ggcatatctg gaaggttatg
     241 gcctcttact tccccggcgc ccgcctgatt aagaccgccg acctggatcc ggctggccgc
     301 tatatattcg tgagccaccc gcacggcgtc atcgccattt ccgactggct ggcatttgcc
     361 acagaggcgc tgggcttctc caaactgttc ccaggcctgg acctgcgctg cgccacgctg
     421 gcttcaaact tctgggtgcc tggtttgcgt gagtacatcc tatcgcacgg catgtgcggc
     481 gtggggcgag acactctggc gcgcgtgctg acaggaaagc cgggccgtgc ggttgtgttg
     541 gtggtgggcg gcgcgtctga ggcgctgttg gcggcggagg gaacttatga cctggtgctg
     601 cgcaaccgca agggctttgt gcgcctggcg ctgcagaccg gcgccagtct ggtgccggtg
     661 ctgtcgtacg gtgagacaga caccttccac acctacatcc cgccgccctg cagccgggcg
     721 gccgcggtca tgaaggtgct gaagcaggtg tttggcttct ccacgcccct gtgctggggc
     781 accggactgt tcgggggctg gggcatgcta gcgctgcagg tgccgctcac tgtggtggtg
     841 ggggcaccca tacaggtgga caaggtgtcc agtcccacgg aggctgaggt ggcggcgctg
     901 cataagacct acacggaggc actgcagaag ctgtgggatg acacagtgga caagtacggc
     961 aagggtgtca agcggccgct ggccatcgtg caatga


[edit]
Categories
//function/biofuels
Parameters
None