Signalling

Part:BBa_K1978002

Designed by: Gregor Hoffmann   Group: iGEM16_Goettingen   (2016-10-14)


TorA-RFP

The TorA-RFP BioBrick consists of a TorA signal linked to RFP, which is fluorescent protein. The TorA signal sequence allows export of fully-folded proteins through the inner membrane via the TAT(Twin-Arginin Translocation) system. This enables export of RFP out of the cell. The TorA sequence codes for a peptide that harbours a twin-arginine motif. This is vital for the recognition by the Tat system. Moreover, an AxA motif is present, which leads to cleavage by the leader peptidase I (Palmer & Berks 2012). MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATA



Usage and Biology

This BioBrick can be used to test a new organism for compatibility with BioBricks using the standart torA signal sequence from E. coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 691
    Illegal AgeI site found at 803
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None