Part:BBa_K1977000

RhtA

RhtA is a membrane protein that is involved in the efflux of threonine and homoserine. Can also export other aminoacids such as proline, serine, histidine, cysteine and aminolevulinic acid.

RhtA exporter, encoded by rhtA gene in E. coli, exports 5-ALA due to its broad substrate specificity. 5-ALA has similar chemical structure to glycylglycine and its physical properties are close to native amino acids carrying uncharged side chains, thus making it possible to be exported via this threonine/homoserine exporter expressed by rhtA gene. [1]

References

[1] Zhen Kang, Yang Wang, Pengfei Gu, Qian Wang, Qingsheng Qi,Engineering Escherichia coli for efficient production of 5-aminolevulinic acid from glucose,Metabolic Engineering,Volume 13, Issue 5,2011,Pages 492-498,ISSN 1096-7176,https://doi.org/10.1016/j.ymben.2011.05.003

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 334
  Illegal AgeI site found at 460
  
• 1000
  COMPATIBLE WITH RFC[1000]