Designed by: Alana Gouveia, Franziska Hameister, Jonas Sindlinger, Maik Schork Group: iGEM16_TU_Darmstadt (2016-10-14)
Colicin E2 DNase trypsin fragmentColicin E2 is a peptide, that is toxic to coliform bacteria. It is one of a group of proteins of this type, it being a nicking endonuclease. It has a corresponding activity inhibiting protein called immunity protein Im2, which is also in the registry under the name.
This part can be created by digesting Colicin E2 with Trypsin.
Figure 1:Structure of Colicin E2 DNase domain derived from a molecular dynamics simulation. Note: this is not actually this part but BBa_K1976048, a 5 amino acids shorter version
The Colicin E2 immunity protein (BBa_K1976027 and BBa_K1976028) can be used in combination with the Colicin E2 DNase domain, or this part as an inhibitor. Since it binds very tightly to the immunity protein, it can also be used in fusing proteins to one another.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 46
- 1000COMPATIBLE WITH RFC
 Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007