Colicin E2 DNase domain "miniColicin"Colicin E2 is a peptide, that is toxic to coliform bacteria. It is one of a group of proteins of this type, it being a nicking endonuclease. It has a corresponding activity inhibiting protein called immunity protein Im2, which is also in the registry under the name BBa_K1976027.
Figure 1:Structure of Colicin E2 DNase domain derived from a molecular dynamics simulation.
The Colicin E2 immunity protein (BBa_K1976027 and BBa_K1976028) can be used in combination with the Colicin E2 DNase. Due to to the high binding affinity of the immunity protein to the DNase domain (Kd ∼ 10− 15 M)2, it can be used to fuse proteins and in combination with a suppression mechanism for the immunity protein as a bacterial killswitch.
|Molecular Weight||15.2 kDa|
|[Data taken created in Snapgene and http://isoelectric.ovh.org/calculate.php]|
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 28
- 1000COMPATIBLE WITH RFC
 Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007
 Wojdyla et al, Journal of Molecular Biology, vol. 417, Issues 1–2, 16 March 2012, Pages 79–94