Composite
Part:BBa_K1976025
Designed by: Patrick Kunzmann Group: iGEM16_TU_Darmstadt (2016-10-13)
Orthogonal pair for amber supression with OMT
Orthogonal pair for amber supression with OMT
An orthogonal pair for O‑methyl‑l‑tyrosine incorporation at the position of the amber stop codon. The pair ist based on the pairs from the "Expanded Genetic Code Measurement Kit" by the iGEM team Austin Texas 2014. The open reading frame (ORF) of the aminoacyl tRNA synthetase was exchanged with the ORF of the OMT tRNA synthtase BBa_K1976020.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1153
Illegal BamHI site found at 1159 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1185
Illegal NgoMIV site found at 1645 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |