Part:BBa_K1975007
PhaB fused to Red Fluorescent Protein
This is a NADPH-Dependent acetoacetyl-CoA reductase (commonly known as PhaB). This enzyme catalyzes the second step in the production of PHB. The reaction it catalyzes is that it reduces acetoacetyl-CoA to (R)-3-hydroxybutyrate. By combining it with the synthetic gene, LPE it can be used in the synthesis of P(LA-co-3HB). This gene is fused with the Red Fluorescence Protein with a small linker containing the restriction site for NgoMIV, so the tag can be cleaved off. The fused RFP is used to visual the expression of the protein. Hereby, the job will be easier to screen for positive transformants after induction (if such is used as in our case). The sequence for PhaB is taken from the BioBrick Part:BBa_K759005 and is optimized to be expressed in Bacillus subtilis.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1177
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 697
Illegal AgeI site found at 1421 - 1000COMPATIBLE WITH RFC[1000]
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