Part:BBa_K1975004
Beta-Ketothiolase fused to Cyan Fluorescent Protein
This is a Beta-Ketoehiolase (commonly known as PhaA). This enzyme catalyzes the first step in the production of PHB. The reaction it catalyzes is two molecules of acetyl-CoA is being putting together to one molecule of acetoacetyl-CoA under the usage of a propanoate that will be converted to acetate. By combining it with the synthetic gene, LPE it can be used in the synthesis of P(LA-co-3HB). This gene is fused with the Cyan Fluorescence Protein with a small linker of 6 bp between. The fused CFP is used to visual the expression of the protein. Hereby, the job will be easier to screen for positive transformants after induction (if such is used as in our case). The sequence for PhaA is taken from the BioBrick Part:BBa_K759004 and is optimized to be expressed in Bacillus subtilis.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1882
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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