Composite

Part:BBa_K1973038

Designed by: José Martín Gómez   Group: iGEM16_UPO-Sevilla   (2016-10-14)


miniTn7BBGm-nahR-Psal-pleD*-glpF

nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. pleD* (BBa_K1973016) and glpF (BBa_K1973026) are expressed from the Psal promoter in the presence of this molecule. Therefore, this construct encodes the genes that are necessary for biofilm induction and glycerol uptake, connecting both activities under the same expression and regulation system. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see https://parts.igem.org/Genome_Integration).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4367
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4367
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4373
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4367
    Illegal BglII site found at 3051
    Illegal BglII site found at 3322
    Illegal BglII site found at 3608
    Illegal BglII site found at 4972
    Illegal BamHI site found at 5584
    Illegal BamHI site found at 6741
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4367
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4367
    Illegal XbaI site found at 4382
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 4804
    Illegal NgoMIV site found at 5999
    Illegal NgoMIV site found at 6146
    Illegal NgoMIV site found at 7078
    Illegal NgoMIV site found at 7153
    Illegal NgoMIV site found at 7210
    Illegal NgoMIV site found at 7624
    Illegal AgeI site found at 6482
    Illegal AgeI site found at 7051
    Illegal AgeI site found at 7567
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1681
    Illegal BsaI site found at 5657
    Illegal SapI.rc site found at 2763


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Categories
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