Part:BBa_K1973028

miniTn7BBGm-nahR-Psal-nasF-glpF

nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. glpF (BBa_K1973026) is expressed from the Psal promoter in the presence of this molecule. nasF (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see https://parts.igem.org/Genome_Integration).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 4367
  Illegal suffix found in sequence at 1
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 4367
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 4373
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 4367
  Illegal BglII site found at 3051
  Illegal BglII site found at 3322
  Illegal BglII site found at 3608
  Illegal BglII site found at 4972
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 4367
  Illegal suffix found in sequence at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 4367
  Illegal XbaI site found at 4382
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NgoMIV site found at 4804
  Illegal NgoMIV site found at 5685
  Illegal NgoMIV site found at 5760
  Illegal NgoMIV site found at 5817
  Illegal NgoMIV site found at 6231
  Illegal NgoMIV site found at 6324
  Illegal AgeI site found at 5658
  Illegal AgeI site found at 6174
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1681
  Illegal SapI.rc site found at 2763