Composite

Part:BBa_K1973024

Designed by: Gabriel Ruiz Romero   Group: iGEM16_UPO-Sevilla   (2016-10-13)


miniTn7BB-Gm-nahR-Psal-xylS2

nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. xylS2 (Bba_K1973002) codifies the modified XylS2 protein from Pseudomonas putida MT1, which is a transcriptional activator of the Pm promoter in response to salicylate. This module provides a tight expression regulation by salicylate thanks to a two-step (NahR and XylS2) system. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see https://parts.igem.org/Genome_Integration).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4367
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4367
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4373
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4367
    Illegal BglII site found at 3051
    Illegal BglII site found at 3322
    Illegal BglII site found at 3608
    Illegal BglII site found at 4972
    Illegal BglII site found at 5493
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4367
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4367
    Illegal XbaI site found at 4382
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 4804
    Illegal NgoMIV site found at 6215
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1681
    Illegal BsaI.rc site found at 5678
    Illegal SapI.rc site found at 2763


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Categories
Parameters
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