Coding
Part:BBa_K1973020
Designed by: Alvaro Escobar Doncel Group: iGEM16_UPO-Sevilla (2016-10-12)
nahR-Psal-xylS2
nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate. xylS2 (BBa_K1973002) is expressed from the Psal promoter in the presence of this molecule. This module provides a tight expression regulation by salicylate thanks to a two-step (NahR and XylS2) system.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
Illegal BglII site found at 1097 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 408
Illegal NgoMIV site found at 1819 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1282
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Categories
Parameters
None |