Composite

Part:BBa_K1972012

Designed by: Shuli Liang   Group: iGEM16_SCUT-China_A   (2016-09-16)


dszBCAD with T7 promoter

We constructed dszBCAD genes with T7 promoter and choose E.coli BL21 as our host cell. When IPTG is added to our system, DszABCD enzymes will be successful expressed and these four enzymes convert dibenzothiophene (DBT) undergoes through three successive oxidation steps and one a hydrolytic step to 2-hydroxybiphenyl (2HBP) by the 4s pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1254
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 498
    Illegal BamHI site found at 2058
    Illegal XhoI site found at 490
    Illegal XhoI site found at 3190
    Illegal XhoI site found at 4009
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 205
    Illegal NgoMIV site found at 2479
    Illegal NgoMIV site found at 2694
    Illegal NgoMIV site found at 2998
    Illegal NgoMIV site found at 3118
    Illegal AgeI site found at 2653
    Illegal AgeI site found at 4197
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2208
    Illegal SapI.rc site found at 3502


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