Plasmid_Backbone

Part:BBa_K1971005

Designed by: KE GUAN YU   Group: iGEM16_BHU_China   (2016-10-20)


pet28a backbone with BsaI site

pet28a backbone with BsaI site

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5130
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 5064
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5130
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5130
    Illegal NgoMIV site found at 237
    Illegal NgoMIV site found at 3284
    Illegal NgoMIV site found at 3444
    Illegal NgoMIV site found at 5032
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 5184
    Illegal SapI.rc site found at 2363


[edit]
Categories
Parameters
None