Part:BBa_K197038

BBb - BseRI/AcuI entry vector

This part is and entry vector in BBb Format. It is flanked by BamHI, BseRI, BglII, and AcuI sites and is used in the 2AB Layered Assembly scheme developed at UC Berkeley. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2472
  Illegal XbaI site found at 333
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2472
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2472
  Illegal XhoI site found at 1486
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2472
  Illegal XbaI site found at 333
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2472
  Illegal XbaI site found at 333
  Illegal NgoMIV site found at 1339
  
• 1000
  COMPATIBLE WITH RFC[1000]