Composite

Part:BBa_K1969022

Designed by: Yijie Lai   Group: iGEM16_SJTU-BioX-Shanghai   (2016-10-12)


ADRB2 with His-tag regulated by GAL1 promoter

The sequence of ADRB2 with His-tag at C terminus is cloned downstream the yeast GAL1 promoter and ended with an ADH1 terminator to generate a median expression cassette in yeast Saccharomyces cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 453
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 453
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1261
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 453
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 453
    Illegal AgeI site found at 70
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 847
    Illegal BsaI.rc site found at 1045


[edit]
Categories
Parameters
None