Regulatory

Part:BBa_K1968000

Designed by: Paolo Marangio   Group: iGEM16_Edinburgh_OG   (2016-10-06)


PA1lac0-1 Phytobrick

Derived from the PA1 promoter of bacteriophage T7, PA1lac0-1 is a LacI-dependent promoter and Isopropyl β-D-1-thiogalactopyranoside (IPTG)-inducible promoter that has been shown to bring about an 8-fold induction in both E. coli and Synechocystis (1).

(1) Guerrero, F., Carbonell, V., Cossu, M., Correddu, D., & Jones, P. R. (2012). Ethylene synthesis and regulated expression of recombinant protein in Synechocystis sp. PCC 6803. PLoS One, 7(11), e50470. doi: 10.1371/journal.pone.0050470

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None