Coding

Part:BBa_K1967021

Designed by: Brendan Largey   Group: iGEM16_Edinburgh_UG   (2016-10-19)


''Deinococcus radiodurans'' DpsMP1

"Deinococcus radiodurans" DNA-binding proteins from starved cells (DpsMP1) is a DNA binding complex used to sequester DNA to protect it from radical oxygen species formed. This BioBrick was first characterized by the Stanford-Brown 2012 iGEM team (BBa_K847002). Here we have codon-optimized the sequence for expression in E. coli and made it compatible for GoldenGate MoClo as a CD-type part.

Deinococcus radiodurans is an atmospheric bacterial species that is known to endure high levels of ionizing-radiation that would be fatal to most other prokaryotic life. Stanford-Brown 2012 demonstrated that this protein may be a factor in D. radioduran's viability in intense conditions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 220
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 134
    Illegal AgeI site found at 703
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None