Coding

Part:BBa_K1966000

Designed by: Brandon Wilbanks   Group: iGEM16_UT-Knoxville   (2016-10-11)


lacPromoter-xylA-xylM

This part contains xylA and xylM from Pseudomonas putida under the control of a lac promoter. xylA and xylM are subunits of P. putida xylene monooxygenase (XO). XO converts a CH3 group of toluene and its derivatives into CH2OH. This is a basic part that is used in BBa_K1966002 with xylM (benzyl alcohol dehydrogenase, BADH) to generate aromatic aldehydes from toluene derivatives.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1798
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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