Coding

Part:BBa_K1965018

Designed by: Nik Franko   Group: iGEM16_Slovenia   (2016-10-17)


CaM(E31Q,E104Q):cLuc

CaM(E31Q,E104Q):cLuc is a genetic fusion of human calmodulin with two introduced mutation E31Q, E104Q and C terminal fragment of split firefly luciferase. Both mutations are located in EF hands of calmodulin and lowers the construct's sensitivity to calcium ion concentration. The part in combination with BBa_K1965014 (nLuc:M13) reconstitutes luciferase activity upon calcium influx into the cell.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 16


[edit]
Categories
Parameters
None