Coding

Part:BBa_K1965016

Designed by: Nik Franko   Group: iGEM16_Slovenia   (2016-10-17)


nTEV:M13

This part is a genetic fusion of N terminal fragment of split TEV protease and a calmodulin (CaM)-binding domain of the skeletal muscle myosin light chain kinase (M13). The part in combination with BBa_K1965017 (CaM(E104Q):cTEV) comprises a split protease system inducible by calcium influx.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 349


[edit]
Categories
Parameters
None