Part:BBa_K1964002

FliC signal sequence

FliC encodes for Flagellin, the main compound of the E. coli flagellum. The 172bp FliC signal sequence directs it to the cell membrane, where it usually gets integrated. In order to utilize this highly regulated mechanism, we generated a FliCD-knockout E. coli strain. This enabled us to fuse the signal sequence to the N-terminal end of our protein of interest, which as a result got exported and secreted into the medium. Therefore, we used it as a possibility to establish a protein-based dependency between S. cerevisiae and E. coli for our system of artificial endosymbiosis. This requires the knockout of an essential gene in yeast and expression as well as export of the corresponding through E. coli.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]