Coding

Part:BBa_K1964001

Designed by: Steffen Lütke   Group: iGEM16_Marburg   (2016-10-14)


Codon optimized GcABC-G1 for Saccharomyces cerevisiae

This part consists of a codon optimized version of GcABC-G1 for Saccharomyces cerevisiae. GcABC-G1 is a monoterpenoid efflux pump from Grossmannia clavigera, which mediates a higher resistance towards Limonene and in general monoterpenoids. The efflux pump is part of the ABC-transporter family and leads to the transfer of monoterpenoids out of the organism into the surrounding enviroment. In the project of the Marburger iGEM-Team 2016 the transporter was used to not only confer a higher resistance towards limonene in S. cerevisiae, but also to secrete the monoterpenoid and final product of the artificial endosymbiotic production line, perillyl alcohol, into the medium.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2932
    Illegal BsaI.rc site found at 34


[edit]
Categories
Parameters
None