Composite

Part:BBa_K1961006

Designed by: Yi-Ting Lin   Group: iGEM16_HSiTAIWAN   (2016-10-11)


Lead ion detector

This part is designed to detect lead ion following the concept of B. It consists of two parts: (1) PbrR generation:this part produces PbrR which can form a dimer of Pb2+ - PbrR in the presence of Pb2+ . (2) RFP expression: this part is controlled by a promoter(BBa_I721001) which has a binding site of dimer of Pb2+ /PbrR. RFP expression can be observed when the dimer binds onto the promoter.

We redo ligation and do function assay.

We tested the survival of E. coli transformed with K1961006 in the Pb2+

alt text

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1281
    Illegal AgeI site found at 1393
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 686

HSiTAIWAN_DESCRIPTION_upload_K1613017.jpeg HSiTAIWAN iGEM16


[edit]
Categories
Parameters
None