Generator

Part:BBa_K1961005

Designed by: Yi-Ting Lin   Group: iGEM16_HSiTAIWAN   (2016-10-03)


PbrR generator expressed by GFP under control of general promoter (J23102)

PbrR is a Pb2+ binding protein. In addition to the PbrR generator with the PTet promoter(K1961004), we designed a PbrR generator (K1961005; also with GFP reporter) under control of general promoter(J23102).

alt text

We compared their GFP expressio to measure their PbrR generation.

We tested the survival of E.coli in tetracycline(TC). E. coli can survival in TC of 1000 ng/ml. In the following figure, it shows that GFP expression of autoregulation(BBa_K1961004) is slower than that with constitutive promoter(BBa_K1961005)

alt text
alt text

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1111


[edit]
Categories
Parameters
None