Part:BBa_K196000

CcdA antidote with the mob promoter (reverse)

Stabilization system : a higher plasmid stability means more proteins.

Principle: in the StabyExpress^TM system, the antidote gene (ccdA: [1] and[2]) is introduced in the plasmid DNA under the control of a weak constitutive promoter, the mob promoter. This promoter comes from a broad host range plasmid (pBHR1). The toxic gene (ccdB) is introduced in the chromosome of the bacteria, which can be furnished by DelphiGenetics. Expression of the poison gene is under the control of a promoter strongly repressed in the presence of the plasmid. When the plasmid is lost, the antidote is degraded and the production of the toxin is induced, causing cell death (for more details, please refer to the "safety" section).For researchers and industries this system offers a great benefit because it is an antibiotic free expression system.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]