Part:BBa_K1957003
HydB (N-terminally tagged) subunit of FeFe Hydrogenase
One of the three subunits that make up FeFe Hydrogenase in Shewanella oneidensis. Specifically, this is the HydB subunit which is the central subunit. This basic part has a strep-tag attached to its N-terminus for consequent protein assays. This unit is one out of the three subunits that can be used to make a the entire FeFe hydrogenase construct.
Restriction site incompatibility, [1000], is with regards to MoClo or Golden Gate cloning. This restriction site was used in Golden Gate cloning experiments in order to assemble the gene cluster into a single expression vector. The Bsal restriction enzyme sites flank the coding sequence, and digestion with Bsal should not disrupt the protein coding sequence. For more details on our Golden Gate cloning strategy and instructions on how to assemble the HydABC gene cluster into a single expression vector please see the NRP-UEA 2016 iGEM teams wiki
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 369
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