Generator

Part:BBa_K195303

Designed by: Tung Chao   Group: iGEM09_NYMU-Taipei   (2009-10-21)


pTet, RBS, neu gene operon and terminator.

The pTet promoter is strong enough to overexpress the neu gene operon.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1718
    Illegal EcoRI site found at 4601
    Illegal EcoRI site found at 4656
    Illegal XbaI site found at 3021
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1718
    Illegal EcoRI site found at 4601
    Illegal EcoRI site found at 4656
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1718
    Illegal EcoRI site found at 4601
    Illegal EcoRI site found at 4656
    Illegal BglII site found at 5014
    Illegal BglII site found at 5185
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1718
    Illegal EcoRI site found at 4601
    Illegal EcoRI site found at 4656
    Illegal XbaI site found at 3021
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1718
    Illegal EcoRI site found at 4601
    Illegal EcoRI site found at 4656
    Illegal XbaI site found at 3021
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1950


[edit]
Categories
Parameters
None