Composite

Part:BBa_K195203

Designed by: Yen-Kuang Chen   Group: iGEM09_NYMU-Taipei   (2009-10-21)


RBS+Lpp-OmpA-linker+alpha-V integrin gene+terminator

Fusion alpha-V integrin gene and Lpp-ompA-Linker(BBa_K103006) together, so alpha-V integrin receptor can anchor on the outer membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2417
    Illegal PstI site found at 2691
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2417
    Illegal PstI site found at 2691
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1516
    Illegal BglII site found at 2319
    Illegal BglII site found at 2973
    Illegal XhoI site found at 2713
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2417
    Illegal PstI site found at 2691
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2417
    Illegal PstI site found at 2691
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1414


[edit]
Categories
Parameters
None