Part:BBa_K1951006

General information about flagellin (FliC protein)

To see an exemple of this biobrick functionality, you can visit our composite part : BBa_K1951009

Representative electron micrographs of nanoparticles produced by D. alaskensis G20. Pd is found on the surface of the cell

This coding sequence codes flagellin C which is a main protein involved for the extracellular flagellar establishment. Flagellar enables bacterial swimming. It has been shown that the flagellin structure from Desulfovibrio can adsorb metallic nanoparticules as gold or platinum.

Design summary

Starting from the Desulfovibrio vulgaris strain Hildenborough flagellin sequence ( http://www.ncbi.nlm.nih.gov/nucleotide/46451220?report=genbank&log$=nucltop&blast_rank=3&RID=WDB55ADB014 ) we have designed a part to produce this protein in E. coli chassis BBa_K1951009. All forbidden restriction sites have been removed. Codons have been optimized for E.coli to allow a maximal transcription level.

Proof of functionality

This sequence has been tested: placed under the control of a strong promoter with a ribosome binding site the sequence has been validated as functional. For further information, go on the following link : BBa_K1951009 design

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1224
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 301
  Illegal AgeI site found at 709
  
• 1000
  COMPATIBLE WITH RFC[1000]