Part:BBa_K1951002

desC, acyl transferase coding sequence

General information

DesC is an acyl transferase of the Desferrioxamine production pathway. It is the enzyme of the third step that transform N-hydroxycadaverin into N-acetyl N-hydroxucadaverine by an Acetyl-CoA dependent manner.

It is also the second step in the production of desferrioxamine B in Streptomyces coelicolor, allowing the transformation of cadeverine into N-hydroxycadaverine.

DesC in the Desferrionxamine B pathway

Design summary

All forbidden restriction sites have been removed.

Codons have been optimized for E.coli to allow a maximal transcription level.

Experiment summary

The following biobrick functionality has been tested by our team in two different composite parts we designed :

• BBa_K1951011 : desABCD cluster - protein production DesC

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]