Coding

Part:BBa_K1947020

Designed by: Jie Tang   Group: iGEM16_NEFU_China   (2016-10-14)


This polypeptide will be fused with the recombinant protein which we want to purify.

There is a highly specific recognition and covalent conjugation between Spytag (a peptide with 13 amino acids) and Spycatcher (a small protein consisting of 138 residues). A protein of interest with a Spytag at its N- or C-terminus is expressed in E. coli and present in bacterial lysate.This part encodes a polypeptide that can combined with Spycatcher, through a covalent bond. In our case this polypeptide will be fused with the recombinant protein which we want to purify.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None