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Part:BBa_K1947014

Designed by: Jie Tang   Group: iGEM16_NEFU_China   (2016-10-13)


We verify the expression effect of Mms13.

In this part, Mms13 and EGFP cDNAs are fused together to produce a fusion protein. Then it can prove whether Mms13 and EGFP can be successfully expressed in AMB-1 by the EGFP florescence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 169
    Illegal NgoMIV site found at 253
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1028


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