Plasmid_Backbone

Part:BBa_K1945011

Designed by: Sharon Lian   Group: iGEM16_CSU_Fort_Collins   (2016-10-21)


pSB1C3 HR Plasmid

This plasmid has a twin https://parts.igem.org/Part:BBa_K1945011 which highlights all the features of this homologous recombination plasmid for use in Synechocystis, sp. PCC 6803. Design and experience details are documented on the other page.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 726
    Illegal suffix found at 754
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 726
    Illegal SpeI site found at 755
    Illegal PstI site found at 769
    Illegal NotI site found at 732
    Illegal NotI site found at 762
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 726
    Illegal XhoI site found at 2512
    Illegal XhoI site found at 3404
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 726
    Illegal suffix found at 755
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 726
    Plasmid lacks a suffix.
    Illegal XbaI site found at 741
    Illegal SpeI site found at 755
    Illegal PstI site found at 769
    Illegal NgoMIV site found at 1237
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1352


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Categories
Parameters
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