Measurement

Part:BBa_K1945003

Designed by: Sharon Lian   Group: iGEM16_CSU_Fort_Collins   (2016-10-14)


PT7 + Luciferase in Homologous Recombination Plasmid

This part acts a reporter that is recognized by the T7 RNA polymerase from bacteriophage T7.

The holding vector is not pSB1C3 but rather our new pSB1C3 based HR plasmid (https://parts.igem.org/Part:BBa_K1945011) for direct integration into the genome of Synechocystis sp. PCC 6803. This composite part was built into the plasmid following standard BioBrick assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 881


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Categories
Parameters
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