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Part:BBa_K1945002

Designed by: Sharon Lian   Group: iGEM16_CSU_Fort_Collins   (2016-10-14)


Homologous Recombination Intermediate for Counter-selection in Synechocystis sp. PCC 6803

This is the sequence that should already be in the genome of Synechocystis sp. PCC 6803 prior to integrating a desired sequence into the organism's genome between neutral site slr0168.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1589
    Illegal PstI site found at 305
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1589
    Illegal NheI site found at 1447
    Illegal PstI site found at 305
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1589
    Illegal BamHI site found at 2
    Illegal BamHI site found at 12
    Illegal XhoI site found at 3057
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1589
    Illegal PstI site found at 305
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1589
    Illegal PstI site found at 305
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2550


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Parameters
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