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Part:BBa_K1945002
Designed by: Sharon Lian Group: iGEM16_CSU_Fort_Collins (2016-10-14)
Homologous Recombination Intermediate for Counter-selection in Synechocystis sp. PCC 6803
This is the sequence that should already be in the genome of Synechocystis sp. PCC 6803 prior to integrating a desired sequence into the organism's genome between neutral site slr0168.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1589
Illegal PstI site found at 305 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1589
Illegal NheI site found at 1447
Illegal PstI site found at 305 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1589
Illegal BamHI site found at 2
Illegal BamHI site found at 12
Illegal XhoI site found at 3057 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1589
Illegal PstI site found at 305 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1589
Illegal PstI site found at 305 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2550
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Categories
Parameters
None |