Part:BBa_K1945001

Homologous Recombination Plasmid for Synechocystis sp. PCC 6803

This is a homologous recombination plasmid for Synechocystis sp. PCC 6803. Any sequence can be cloned into the plasmid using standard iGEM BioBrick assembly protocols because the prefix and suffix are moved in between the neutral site slr0168. Transformation into the organism will allow recombination of the plasmid into slr0168.

The plasmid map on iGEM's page is not correct because the composite part is between VF2 and VR. See our design page for more information and the accurate plasmid map.

This part has a twin called https://parts.igem.org/Part:BBa_K1945011 to categorize it as a plasmid. It also has the complete sequence including where these new features integrate into the standard pSB1C3 plasmid. To perform homologous recombination, the organism should already have a strain with this sequence (https://parts.igem.org/Part:BBa_K1945011) to complete the counter-selection method.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 734
  Illegal suffix found in sequence at 764
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 734
  Illegal SpeI site found at 765
  Illegal PstI site found at 779
  Illegal NotI site found at 740
  Illegal NotI site found at 772
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 734
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 734
  Illegal suffix found in sequence at 765
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 734
  Illegal XbaI site found at 749
  Illegal SpeI site found at 765
  Illegal PstI site found at 779
  Illegal NgoMIV site found at 1269
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1384