Device

Part:BBa_K1945000

Designed by: Sharon Lian   Group: iGEM16_CSU_Fort_Collins   (2016-10-14)


PSigA and PTic2op AND GATE

AND gate controlling firefly luciferase expression under a T7 promoter using a split T7 RNA polymerase system. The T7 fragments are under the control of a constitutive promoter SigA and IPTG-inducible promoter Tic2op, both which are native to Synechocystis sp. PCC 6803.

This part is in the the modified pSB1C3 plasmid (https://parts.igem.org/Part:BBa_K1945011) for integration into the genome of this cyanobacterium. It is located between prefix and suffix sequences.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 734
    Illegal suffix found in sequence at 5685
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 734
    Illegal SpeI site found at 5686
    Illegal PstI site found at 5700
    Illegal NotI site found at 740
    Illegal NotI site found at 5693
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 734
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 734
    Illegal suffix found in sequence at 5686
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 734
    Illegal XbaI site found at 749
    Illegal SpeI site found at 5686
    Illegal PstI site found at 5700
    Illegal NgoMIV site found at 6176
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 6291
    Illegal SapI.rc site found at 4831


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Categories
Parameters
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