Part:BBa_K1941000

PCP fused to Mxi1 domain

RNA binding protein PCP fused to the transcriptional repression domain Mxi1.

Usage and Biology

MAX-interacting protein 1 (Mxi1), is a transcriptional repressor of the c-Myc promoter. Initially found in mammalian cells, it is also active in yeast cells. PCP is a RNA binding protein that can be recruited by a small RNA sequence, PP7. The fusion between these two proteins gives the possibility to Mxi1 to be recruited where the PP7 sequence is. For example, when producing PCP-Mxi1 along with BBa_K1941003, a scaffold RNA (SCRNA) with the PP7 sequence fused to it, the repressor is recruited to the scRNA and can repress CYC1 promoter.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 77
  Illegal BglII site found at 333
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]