Part:BBa_K1938004
cld
Chlorite dismutase
Catalyzes the heme-dependent decomposition of chlorite to O2 and chloride with high efficiency and specificity. Used to detoxify chlorite, a by-product of the reduction of perchlorate, a primarily anthropogenic pollutant, in perchlorate-respiring bacteria.
Usage and Biology
The Leiden 2016 team used this part, but did not manage to build it. Exeter iGEM 2018 improved function by managing to express it and by confirming expression through a Western blot and assaying function by measuring oxygen production of Cld in the presence of chlorite, as shown below.
We've resubmitted this part under the part number BBa_K2695010 (https://parts.igem.org/Part:BBa_K2695010)
Figure 5: Western blot for Cld constructs
Expected molecular weights (kDa): A = 32.97 | B = 32.99 | C = 32.96 | D = 31.20
The Western Blot displayed expression of chlorite dismutase for D. aromatica and therefore we have proved expression of our new part.
WT = Wild Type E. coli (strain: BL21 (DE3))
Out of the parts collection of chlorite dismutase enzymes, D. aromatica cld was the most effective at producing oxygen. Therefore we have shown that our part is functional in E. coli.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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