Part:BBa_K1937001
pSBBsOK-mini, a small replicative plasmid for Bacillus subtilis
Part: BBa_K1937001 (pSBBsOK-mini)
(Chassis E. coli, carrier plasmid pSB1C3, part destined for use in Bacillus subtilis) Length: 5857 bp
This part is an expression vector for Bacillus subtilis. It is replicative both in E.coli and B.subtilis. It has an ampicillin resistance for cloning in E.coli and kanamycin resistance for selection in B. subtilis.
This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France).
We successfully transformed E. coli and selected clones on ampicillin. Likewise, we successfully transformed B. subtilis and selected clones on kanamycine. The plasmid sequence has been entirely verified by sequencing. The capacity to sub-clone in this new backbone has been demonstrated (part BBa_K1937004, BBa_K1937006, BBa_K1937008 and BBa_K1937010).
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 257
Illegal suffix found in sequence at 285 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 257
Illegal NheI site found at 279
Illegal SpeI site found at 286
Illegal PstI site found at 300
Illegal NotI site found at 263
Illegal NotI site found at 293 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 257
Illegal BglII site found at 4814
Illegal BamHI site found at 330 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 257
Illegal suffix found in sequence at 286 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 257
Illegal XbaI site found at 272
Illegal SpeI site found at 286
Illegal PstI site found at 300
Illegal NgoMIV site found at 1877
Illegal AgeI site found at 4425
Illegal AgeI site found at 5387 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 55
Illegal BsaI.rc site found at 1701
Illegal BsaI.rc site found at 3140
Illegal BsaI.rc site found at 5656
Illegal SapI site found at 618
Illegal SapI.rc site found at 4638
//chassis/prokaryote/ecoli
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