Part:BBa_K1936002

tetR-Pif6 for yeast

Usage and Biology

The TetR (tetracyclin Repressor) is originally found in bacteria and encodes natively for tetracycline resistance. [1] In scientific use it is usually fused to VP16 from herpes simplex virus, which acts as a transactivator and recruits RNA Polymerase to express the related gene. [2] TetR binds the tetO motiv in the construct upstream of a promoter and the gene(s).

PIF6 is fused to tetR that binds the tetO motif in the designed construct upstream of a minimal promoter and the designed construct/gene. PIF6 is the interacting factor or Phytochrome B (PhyB), which is a photoreceptor from Arabidopsis thaliana. When Phy B is activated with red light it can be recruited and bound to PIF6. [3]

Biobrick in combination with PhyB-VP16:
In red light, PhyB-VP16 is recruited to PIF6 at the promoter site, which activates the expression of the genes. Upon absorption of far red light the interaction of Phyb and PIF6 is terminated, resulting in silencing of the gene. [3]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Christoph F. Beck, Rupert Mutzel, Jordi Barbe and Wolfram Müller (1981) A Multifunctional Gene (tetR) Controls Tn10-Encoded Tetracycline Resistance. Journal of Bacteriology, May 1982, p. 633-642, 0021-9193/82/050633-10502.00/0
[2] Eloi Gari, Lidia Piedrafita, Marti Aldea and Enrique Herrero (1996) A Set of Vectors with a Tetracycline-Regulatable Promoter System for Modulated Gene Expression in Saccharomyces cerevisiae. Yeast vol. 13: 837-848 (1997)
[3] Konrad Müller, Matias D Zurbriggen and Wilfried Weber(2014) Control of gene expression using a red- and far-red light-responsive bi-stable toggle switch. Doi:10.1038/nprot.2014.038 February 2014