Regulatory
Part:BBa_K1935022
Designed by: Emilie Gounin Group: iGEM16_Bordeaux (2016-10-14)
SET-16 promoter in Caenorhabditis elegans
Ceanorhabditis elegans is an organism with a free-methylated cytosine genome. We looked for a worm’s promoter able to be regulated as mammalian CpG island.
pSET-16 is a promoter of Caenorhabditis elegans classified as a HOT (Highly Occupied Target) regions. HOT regions have a rich G-C content that makes them similar to mammalian CpG islands.
Some HOT promoters have been mentioned by Chen and one of them, SET-16 (chromosome 3: 13643462-13643761), controls a non-essential gene. That’s why we chose it as a target to test epiCRISPR whithout killing the worm.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |