Composite

Part:BBa_K1935014

Designed by: Jean DESCARPENTRIE   Group: iGEM16_Bordeaux   (2016-10-13)


White Collar 1 under the control of pU6 promoter in C.elegans (pU6::WC1)

This construction was made to express White Collar 1 in Caenorhabditis elegans and activate pFRQ under blue light exposure (450 nm).

Usage and Biology

See BBa_K1935006 for more details about pU6 "regulatory sequence of C.elegans".
See BBa_K1935003 for more details about White collar-1 (WC-1) "photoreceptor in Neurospora tetrasperma".


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2383
    Illegal BglII site found at 3019
    Illegal BamHI site found at 2664
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1946
    Illegal BsaI site found at 2290
    Illegal BsaI site found at 2743
    Illegal SapI.rc site found at 2885
    Illegal SapI.rc site found at 3184


[edit]
Categories
Parameters
None