Generator

Part:BBa_K193211

Designed by: Nao Nakatani   Group: iGEM09_Tokyo_Tech   (2009-10-14)

EGFP reporter on constitutive promotor

EGFP reporter on constitutive promotor (T7 promoter BBa_I719005).

Figure. Checked Antifreeze protein expression by SDS-PAGE. More information see [http://2009.igem.org/Team:Tokyo_Tech/Antifreeze#Experiments Experiments]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 786
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 786
    Illegal NheI site found at 804
    Illegal NotI site found at 816
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 786
    Illegal BamHI site found at 582
    Illegal XhoI site found at 483
    Illegal XhoI site found at 825
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 786
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 786
  • 1000
    COMPATIBLE WITH RFC[1000]


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