Composite

Part:BBa_K1925005

Designed by: Zi Yue Wang   Group: iGEM16_Fudan   (2016-10-11)


3xtRNA tandom repeat(tRNA array)

In order to express three shRNAs simutaneously within only one transcript,we design design artificial tandom series tRNAs to imitate natural tandom series tRNAs,in which we design restriction enzyme sites between each tRNAs.Using these restriction enzyme sites we can easily insert shRNA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 195
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 30
  • 1000
    COMPATIBLE WITH RFC[1000]


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