Composite
U6 array

Part:BBa_K1925004

Designed by: Zi Yue Wang   Group: iGEM16_Fudan   (2016-10-11)


3xtetO-U6 tandom repeat(U6 array)

To express three shRNAs simultaneously, we originally design tandem serial Tet-O-U6 promoters flanked with engineering restriction enzyme sites. shRNAs could be easily inserted through digestion and ligation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 717
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 373
    Illegal BamHI site found at 751
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None