Composite
U6 array
Part:BBa_K1925004
Designed by: Zi Yue Wang Group: iGEM16_Fudan (2016-10-11)
3xtetO-U6 tandom repeat(U6 array)
To express three shRNAs simultaneously, we originally design tandem serial Tet-O-U6 promoters flanked with engineering restriction enzyme sites. shRNAs could be easily inserted through digestion and ligation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 717
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 373
Illegal BamHI site found at 751 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |