Coding

Part:BBa_K1923005

Designed by: Tianyang Mao   Group: iGEM16_Tsinghua   (2016-10-14)


Thymidine kinase encoding gene

This basic part encodes an enzyme called thymidine kinase can function as a selectable and counterselectable marker gene in yeast. In certain medium condition TK positive yeast will survive while in another medium condition only TK negative yeast can grow [1].

For more information, please refer to the Wiki page of Team Tsinghua.

Reference:
[1] Alexander, W. G., Doering, D. T., & Hittinger, C. T. (2014). High-efficiency genome editing and allele replacement in prototrophic and wild strains of saccharomyces. Genetics, 198(3), 859–866.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None