Coding

Part:BBa_K1923001

Designed by: Tianyang Mao   Group: iGEM16_Tsinghua   (2016-10-14)


C2c2 encoding gene

Class 2 type VI-A CRISPR-Cas effector C2c2 is a programmable RNA-guide RNase. In vitro biochemical analysis shows that C2c2 is guided by a single crRNA and can be programmed to cleave ssRNA targets carrying somplementary protospacers. In bacteria, C2c2 can be programmed to knock-down specific mRNAs [1].

For more information, please refer to the Wiki page of Team Tsinghua.

Reference:
[1] Abudayyeh O O, Gootenberg J S, Konermann S, et al. C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector[J]. Science, 2016: aaf5573.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 572
    Illegal BglII site found at 1196
    Illegal BglII site found at 1592
    Illegal BglII site found at 1883
    Illegal BglII site found at 1973
    Illegal BglII site found at 3134
    Illegal BglII site found at 3221
    Illegal BamHI site found at 4216
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4173
    Illegal NgoMIV site found at 4192
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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