C2c2 encoding gene
Class 2 type VI-A CRISPR-Cas effector C2c2 is a programmable RNA-guide RNase. In vitro biochemical analysis shows that C2c2 is guided by a single crRNA and can be programmed to cleave ssRNA targets carrying somplementary protospacers. In bacteria, C2c2 can be programmed to knock-down specific mRNAs .
For more information, please refer to the Wiki page of Team Tsinghua.
 Abudayyeh O O, Gootenberg J S, Konermann S, et al. C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector[J]. Science, 2016: aaf5573.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 572
Illegal BglII site found at 1196
Illegal BglII site found at 1592
Illegal BglII site found at 1883
Illegal BglII site found at 1973
Illegal BglII site found at 3134
Illegal BglII site found at 3221
Illegal BamHI site found at 4216
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 4173
Illegal NgoMIV site found at 4192
- 1000COMPATIBLE WITH RFC